Extended Data Fig. 4: Expression patterns of ZmMYB genes under drought and multiple sequence alignment of representative MYB proteins from different clades.
From: Natural variation in ZmDapF1 enhances maize drought resilience
a, Heat map of gene expression levels of ZmMYBs in B73 in response to drought treatment, based on RNA-sequencing analysis quantified by FPKM (fragments per kilobase million). Drought treatment was applied until relative leaf water content reached ~70% (WS1) and ~58% (WS2). b, Reverse transcirption-quantitative PCR (RT-qPCR) analysis of expression levels of drought-induced ZmMYBs in B73 under well-watered and drought-stress conditions. Data were obtained from three independent replicates, and for each replicate leaves from four plants of each genotype were mixed and analyzed. Data represent the means ± s.d., and statistical significance was determined by a two-sided t-test. c, Phylogenetic relationships of the ZmMYB and AtMYB proteins. The phylogenetic tree was constructed using the Neighbor-Joining method with MEGA 7 software. d, ZmMYB121 and ZmMYB131 protein contain the C2 motifs, which are characteristic of most R2R3-MYB repressors. The conserved R2 and R3 MYB domains are indicated and the C2 motifs are framed.
