Extended Data Fig. 5: ZmMYB121 preferentially binds to ZmDapF1-Hap2 promoter.
From: Natural variation in ZmDapF1 enhances maize drought resilience
a, No suppression of cell growth was observed on the yeast cells transformed with ZmMYB121-mut compared with those transformed with the AD empty vector. b, Other five ZmMYB proteins did not show differently inhibition activity on the ZmDapF1-Hap1 and ZmDapF1-Hap2 promoters in yeast one-hybrid assays. c, Competition test in EMSA showing that the ZmDapF1-Hap2 sequence strongly competes with the ZmDapF1-Hap1 sequence for ZmMYB121 binding at both MBS1 (MYB binding site 1) (up) and MBS2 (down). The binding intensities were quantified using ImageJ software, and relative values are shown on the images. Similar results were obtained in three independent experiments.
