Extended Data Fig. 7: ZmMYB121 confers drought resistance through regulation of ZmDapF1.
From: Natural variation in ZmDapF1 enhances maize drought resilience
a, Drought resistance test of ZmMYB121-OE and zmdapf1-KO plants. Left panel: representative images taken before and after drought treatment; right panel: statistical analysis of survival rates. Twelve plants of each genotype were tested in each experiment, and six replicates were conducted. Significance was determined by one-way ANOVA (Tukey’s test). Scale bar, 5 cm. b, Schematic diagram illustrating the CRISPR-targeted mutations in zmmyb121-KO plants. c, Representative images taken before and after drought treatment in drought resistance test of zmmyb121-KO plants. Scale bar, 5 cm. d, Statistical analysis of survival rates in drought resistance test of zmmyb121-KO plants. Fifteen plants of each genotype were tested in each experiment, and four replicates were conducted. e, Relative expression levels of ZmDapF1 in zmmyb121-KO plants under WW and WS conditions. Data were obtained from three independent replicates; for each replicate, leaves from three plants of each genotype were mixed and analyzed. Values represent means ± s.d., and statistical significance was determined by a two-sided t-test (d-e).
