Fig. 3: Experimental mesocosm design for low-light challenge.
From: Hybridization and low-light adaptability in California eelgrass (Zostera spp.)
a–c, The mesocosm, maintained outdoors with flow-through seawater, contained transplanted hybrid and Z. marina plants growing in a common garden. At the end of each sampling day before, during and after dark treatment, plants were removed, and leaf (L) and rhizome (R) tissues were collected for nucleotide extraction (a). The tank was fed with continuously flowing seawater at ambient ocean temperature, and air was bubbled in along the central axis. Experimental time points 1–4 and replicates a–c are shown (b). The experimental timeline contained an acclimation period, a light sample (t1) and two low-light samples at three days (t2) and five days (t3), after which the tank was uncovered, and a recovery time point (t4) was taken after a day. All tissue samples were collected just prior to sunset (c).
