Fig. 1: BSL1 phosphatase activity is essential for viability in Chlamydomonas.
From: Mitotic entry is controlled by the plant-specific phosphatase BSL1 and cyclin-dependent kinase B
a, Diagram of the ProBSL1::BSL1-Venus::paroR construct showing the locations of the bsl1-1 mutation, the insertion in bsl1-ko and the D619N substitution. b, Anti-FLAG immunoblot (IB) showing BSL1–VF and BSL1(D619N)–VF in the bsl1-1 and bsl1-ko backgrounds. WT, wild type. c, Growth assay of the indicated strains. Cells grown at 21 °C were spotted in fivefold serial dilutions and then grown at 21 °C or 33 °C. Note the lethality of bsl1-1 and of bsl1-1::BSL1D619N-VF at 33 °C. d, Anti-FLAG and anti-BSL1 immunoblots showing the expression of BSL1, BSL1(L46P)–VF and BSL1–VF, and the absence of endogenous BSL1 in bsl1-ko. The experiment was performed once.
