Extended Data Fig. 10: Mutations of CDKB1-T14/Y15 and BSL1 alter the sensitivity to DNA replication stress.
From: Mitotic entry is controlled by the plant-specific phosphatase BSL1 and cyclin-dependent kinase B
a, Cells expressing CDKB1AF are hypersensitive to the polymerase inhibitor aphidicholin, but not to the DNA-damage-inducing drug zeocin. cdkb1-1 cells complemented with either wild type CDKB1-mCherry (CDKB1) or unphosphorylatable CDKB1AF-mCherry (CDKB1AF, three independent clones). The density of each culture was normalized to OD750 ~ 0.4 and cells were spotted in 5-fold serial dilutions on TAP agar plates containing 50 μM aphidicholin or 2 μg/ml zeocin and incubated at 21 °C and 33 °C. b, Sensitivity of the bsl1-1 mutant to HU. Cells were spotted (5-fold serial dilutions) on TAP agar plates, and plates supplemented with 1 mM HU, and incubated at at 21 °C.
