Extended Data Fig. 9: Effect of ABA and GR24 treatment.
From: Carotenoid biosynthesis drives root plasticity through aerenchyma and iron plaque formation in rice
a-b, Iron plaque induction assay in Kitaake (a) and psy2-3 mutant (b) in response to ABA and (rac)-GR24 treatment. Red arrowheads indicate iron plaque forming adventitious roots. Scale bars, 2 cm. c, Aerenchyma (%) in response to ABA and (rac)-GR24 treatment in 5 cm-long adventitious roots of 2-week-old plants at 1-2 cm and 3-4 cm from the tip (n = 4 root cross sections). d, Aerenchyma (%) in response to (+)-GR24 treatment in 7 cm-long adventitious roots of 3-week-old plants at 3-4 cm and 5-6 cm from the tip (n = 4 root cross sections). In (c) and (d), data are shown as means ± SE and P values were obtained using the two-tailed Student’s t-test. e-g, Root length (e), root dry weight (f), and shoot length (g) in response to ABA and (rac)-GR24 treatment. Data are shown as means ± SE (n = 5 roots). h-i, Root hair density (h) and Root hair length (i). Data are shown as means ± SE (n = 9 roots). Different letters in (e), (f) and (g) indicate significant differences at P < 0.05, as determined by two way analysis of variance followed by Tukey test. Measurements in (e), (f), (g), (h), and (i) were conducted in 3-week-old plants. Different letters in (h) and (i) indicate significant differences at P < 0.05, as determined by Kruskal-Wallis test followed by Dunn test.
