Extended Data Fig. 3: Ionic currents conferred by ICA expression are not significantly affected by anions in bath solutions and are dependent on cation concentrations.
a-d, Current-voltage (I-V) relationships for water-injected oocytes and oocytes expressing AtICA1 (a), AtICA2 (b), AtICA3 (c), and AtICA4 (d) were measured in solutions with different concentrations of Ba2+ (0, 2, 10, and 50 mM). n = 8 (a,b,d). n = 8 for 0 mM Ba2+, n = 7 for 2 mM Ba2+, n = 6 for 10 mM Ba2+ and 50 mM Ba2+ (c). e-h, I-V relationships for water-injected oocytes and those expressing AtICA1 (e), AtICA2 (f), AtICA3 (g), and AtICA4 (h) were evaluated in solutions with varying concentrations of K+ (0, 2, 10, and 50 mM). n = 8 for 0 and 10 mM K+, n = 9 for 2 mM K+, and 50 mM K+ (e); n = 8 for 0 and 2 mM K+, n = 9 for 10 mM K+, n = 10 for 50 mM K+ (f); n = 8 for 0 mM K+, n = 9 for 2 mM and 50 mM K+, n = 6 for 10 mM K+ (g); n = 8 for 0 and 50 mM K+, n = 6 for 2 mM K+, n = 9 for 10 mM K+ (h); i-l, I-V relationships for water-injected oocytes and oocytes expressing AtICA1 (i), AtICA2 (j), AtICA3 (k), and AtICA4 (l) were measured while perfused with solutions containing CaCl2, Ca(NO3)2, or Ca-(Glu)2, respectively. n = 8 (i-l). m-p, I-V relationships for water-injected oocytes and oocytes expressing AtICA1 (m), AtICA2 (n), AtICA3 (o), and AtICA4 (p) were measured in solutions containing KCl, KNO3, or K-Glu, respectively. n = 8 (m-p). q, I-V relationships for oocytes expressing AtICA1 were measured in solutions containing 10 mM CaCl2 with pH 5.6 or pH 7.4. n = 6. All data are mean ± s.d. Similar results were obtained from more than three independent batches of oocytes.
