Extended Data Fig. 8: ica1/2/3/4 quadruple mutants exhibit defective whole-cell Na⁺ currents in root epidermal cells and reduced Ca2+ influx in root surface.
a-d, ica1/2/3/4 quadruple mutants exhibit defective whole-cell Na⁺ currents in root epidermal cells. Typical whole-cell recordings of Na⁺ currents in WT, ica1/2/3/4-1, and ica1/2/3/4-2 root epidermal cells under normal conditions (a). The current-voltage (I-V) relationships derived from these recordings are shown in (b). Similar analyses were conducted for WT, ica1/2/3/4-1, and ica1/2/3/4-2 root epidermal cells under NaCl stress (c and d). e-h, The whole-cell K⁺ currents in WT, ica1/2/3/4-1, and ica1/2/3/4-2 root epidermal cells are comparable. Typical whole-cell recordings of K⁺ currents in WT, ica1/2/3/4-1, and ica1/2/3/4-2 root epidermal cells under normal conditions (e). The current-voltage (I-V) relationships derived from these recordings are shown in (f). Similar analyses were conducted for WT, ica1/2/3/4-1, and ica1/2/3/4-2 root epidermal cells under NaCl stress (g and h). Data are mean ± s.e.m. (b,d,f,h), n = 8 cells. Three biological repeats were performed with similar results.
