Extended Data Fig. 8: Pm4b and Pm4a phenotypes and SvrPm4 candidate gene expression levels in four B. g. tritici reference isolates.

(A) Virulence phenotypes of reference isolates CHE_96224, CHE_94202, ISR_7 and GBR_JIW2 on the near isogenic lines ‘W804/8*Fed’ (Pm4b) and ‘Khapli/8*CC’ (Pm4a). Pictures were taken eight days post inoculation. (B) Expression levels of SvrPm4 candidate genes Bgt-51526, BgtAcSP-30824 and BgtAcSP-31023 in the four B. g. tritici reference isolates CHE_96224, CHE_94202, ISR_7 and GBR_JIW2. Expression levels are shown as RPKM values at 48 h post infection on the susceptible wheat cultivar ‘Chinese Spring’. Values of three biological replicates are shown as yellow circles.