Extended Data Fig. 4: Western blots of different AvrPm4 and Pm4 constructs.
(A) Protein levels of the different constructs used for the split luciferase experiment (Fig. 3a, b). The same membrane was imaged for 1 s (i) or 30 s (ii) exposure time. Ponceau staining below the blots shows equal protein loading. (B) Detection of full-length mTurquoise-AvrPm4 (65 kDa) co-expressed with either mCherry-Pm4b-V1 (90 kDa) or Venus-Pm4b-V2 (113 kDa) in the N. benthamiana samples used for the co-localisation experiments. The unlabelled bands observed in the WB (indicated with an asterisk) correspond to partial degradation products of the target protein and/or free fluorophore. (C) Coomassie Brilliant Blue G-250 staining and western blot of purified MBP-tagged proteins used in the in-vitro kinase assay. All immunoblotting experiments have been performed twice with similar results.
