Extended Data Fig. 10: StWRKY75 is induced in host microenvironment of potato leaves and contributes to resistance by modulating multi-layered pathway.
a, The representative images showing spatial visualization of StWRKY75. The white dotted lines represent the boundary between the inoculated and the non-inoculated regions as defined by the spatial distribution of zoospore droplets. Scale bar, 100 μm. b, Box plot showing the expression level of StWRKY75 in Mock, PTCs and SPCs. Pairwise comparisons within each time point were performed using two-tailed Mann-Whitney U test (ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001). c, d, Transcript levels of StWRKY75-RNAi (c) and overexpression plants (d) were measured by RT-qPCR and normalized with endogenous StELF1. Bars represent the mean ± s.d. (n = 3 biological replicates). The asterisks indicate significant differences as assessed by one-way ANOVA (with Tukey’s test) (****P < 0.0001). e, f, Relative biomass of P. infestans was determined by qPCR of P. infestans genomic DNA normalized to potato genomic DNA at 4 dpi. Bars represent the mean ± s.d. (n = 3 biological replicates). The asterisks indicate significant differences as assessed by one-way ANOVA (with Tukey’s test) (***P < 0.001, ****P < 0.0001). This experiment was repeated three times with similar results. g, h, P. infestans infection assay on StWRKY75-RNAi (g) and overexpression plants (h). Plants were photographed at 4 dpi. Scale bar, 1.5 cm. i, Volcano plot depicting the DEGs resulting from the overexpression of StWRKY75 compared with WT. j, Bulk RNA-seq of Desiree WT and StWRKY75 overexpression plants treated with water (Mock) or P. infestans strain 88069 at 12 hpi. DEGs (adjusted P < 0.05, |log2-adjusted fold change| > 1) between Desiree WT and StWRKY75 overexpression after infection are shown. k, Transcript levels of indicate genes were measured by RT-qPCR and normalized with endogenous StELF1. Bars represent the mean ± s.d. (n = 3 biological replicates). The asterisks indicate significant differences as assessed by one-way ANOVA (with Tukey’s test) (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). In b–f and k, exact P values are shown in source data with 95% confidence intervals. In i and j, Adjusted P value (Benjamini-Hochberg method) was calculated with two-tailed Wald test implement in DESeq2 package.
