Extended Data Fig. 3: Spatiotemporal transcriptomic atlas of all samples.

a, The UMAP visualization (upper panel) and spatial visualization (lower panel) of the unsupervised clustering results for cells from each separated time point, each dot represented a segmented cell and the color indicated cluster identity. b, Statistics of UMI (left panel) and gene counts (right panel) of each cluster. The box plots displaying the 25th to 75th percentiles, with a center line representing the median and whiskers extending to the minimum and maximum values within 1.5 times the interquartile range. The sample sizes for these box plots are provided in Supplementary Table 6. c, Spatial visualization of clusters showcasing the align with experimental design. Clusters were labeled as infected or non-infected based on their location within the actual P. infestans zoospore droplet-treated area. Each dot represented a segmented cell, with red, blue and green colors indicating clusters from inoculated, non-inoculated and mock regions, respectively. d, Statistics of cell number in each cluster. e, UMAP visualization presenting the clusters categorized as Inoculated, Non-inoculated and Mock-treated parts (left panel) and cell types (right panel). Each dot represented a segmented cell, red color: Inoculated cells; blue color: Non-inoculated cells; green: Mock-treated cells.