Extended Data Fig. 7: Characterization of cure1 cure2 cure3 triple mutants and their phenotypes.
From: A biosynthetic gene cluster for three post-chorismate pathways in Arabidopsis
(a) cure1 cure2 cure3 triple mutants generated via CRISPR/Cas9 gene editing. One guide RNA (red triangle) was used for each gene, the sequences of which are shown in green in the WT. The underlined bases indicate the PAM site (NGG in this study). The mutations within each CURE gene are indicated in red and were confirmed by PCR-product sequencing. (b) Root tip of cure1 cure2 cure3 triple mutants and WT at 5-day-old seedling stage, stained by propidium iodide and observed by confocal microscopy. One representative of five biological replicates is shown for each line. Scale bar = 20 µm. (c) Twelve-day-old cure1 cure2 cure3 triple mutants seedlings, vertically grown in vitro. Scale bar = 1 cm. (d-e) Primary root length (d) and lateral root density (e) for cure1 cure2 cure3 triple mutants and WT plants shown in (c). Data are shown as mean ± SD. The number of biological replicates is shown in the figure. P values were estimated by two-tailed Student’s t-test. (f) The above-ground part of cure1 cure2 cure3 triple mutants and WT plants grown in soil for one month. One representative of nine biological replicates is shown for each line. Scale bar = 1 cm. (g-h) The root systems of cure1 cure2 cure3 triple mutants and WT plants grown in soil for one month, side view (g) and bottom view (h), respectively. One representative of nine biological replicates is shown for each line (g) and three representatives of nine biological replicates are shown for each line (h). Scale bar = 1 cm.
