Extended Data Fig. 8: Searching for CURE1, CURE2, DCMT1, UGT76E9 and DCMT2 homologues in 35 plant species.
From: A biosynthetic gene cluster for three post-chorismate pathways in Arabidopsis
(a) Summary of homologues for each of the five enzymes obtained by protein BLAST. The homologous proteins and their phylogeny are shown in Supplementary Figs. 26–29. The cut-off identity values, that is, 70%, 60%, 65% and 60% identity for CURE1 and CURE2, DCMT1, UGT76E9, and DCMT2, respectively, are based on the phylogeny of the homologous proteins in A. thaliana (see b-e). Twenty-four other Brassicaceae species include Arabidopsis helleri (Ah), Alyssum linifolium (Alyli), Arabidopsis lyrata (AL), Brassica oleracea capitata (Bol), Brassica rapa (Brara), Boechera stricta (Bostr), Caulanthus amplexicaulis (Caamp), Capsella grandiflora (Cagra), Capsella rubella (Carub), Camelina sativa (Csa), Descurainia sophioides (Desop), Diptychocarpus strictus (Distr), Euclidium salsugineum (Thhalv), Euclidium syriacum (Eusyr), Iberis amara (Ibeam), Isatis tinctorial (Isati), Lunaria annua (Luann), Lepidium sativum (Lesat), Malcolmia maritima (Mamar), Myagrum perfoliatum (Myper), Rorippa islandica (Roisl), Schrenkiella parvula (Sp), Stanleya pinnata (Stapi), and Thlaspi arvense (Thlar). Other abbreviations used in the phylogenetic analysis include Cleome violacea (Clevi), Vitis vinifera (Vitiv), Zea mays (Zm), Oryza sativa (LOC). (b) Maximum likelihood (ML) tree of 11 CURE homologs in A. thaliana. Protein identity shared with CURE1 (left) and CURE2 (right) is shown. P: likely pseudogene. (c) Part of the reported ML tree of SABATH family proteins in A. thaliana25. Protein identity shared with DCMT1 is shown. (d) Part of the reported ML tree of UGT76E family proteins in A. thaliana58. (e) Part of the reported ML tree of SABATH family proteins in A. thaliana25. Protein identity shared with DCMT2 is shown.
