Extended Data Fig. 5: JA upregulates carbohydrate content in dormant buds and activates GhSUT4 and GhCYCD2;1 promoters.
From: GhZAT11 triggers wound-activated bud growth by accelerating sugar transport and cell division
a, b, JA increases carbohydrate substance content in dormant buds at 3 DAT. MeJA-treated buds had deeper PAS staining. Scale bars, 100 μm. The mean gray value of PAS staining was measured using ImageJ (n = 5 buds). c-f, JA enhances GhSUT4 (c, d) and GhCYCD2;1 (e, f) promoter activity. N. benthamiana leaves expressing GhSUT4pro:LUC and GhCYCD2;1pro:LUC were treated with 100 μM MeJA for 6 hours before CCD imaging. Quantification of luciferase activities were measured (d, f). DMSO served as control (n = 5 independent leaves). g-j, SHAM suppresses GhSUT4 and GhCYCD2;1 promoter activity induced by wounding. N. benthamiana leaves expressing GhSUT4pro:LUC (g, h) and GhCYCD2;1pro:LUC (i, j) were wounded, treated with 1 mM SHAM for 6 h, and imaged by CCD. Quantification of luciferase activities were measured (h, j). Data in panel h: n = 6 independent leaves. Data in panel j: n = 5 independent leaves. All data in this figure are shown as mean ± s.d. Different letters in panels h and j indicate significant differences according to one-way ANOVA with LSD’s multiple comparisons test (P = 0.0001). Panels b, d, f: two-sided Student’s t-test (*P < 0.05, **P < 0.01).
