Extended Data Fig. 3: Inhibition of autophagosome formation by 3-MA did not block PME.

a, Schematic diagram of the 3-MA treatment experiments. Treatment ①: the pollen grains from a vacuole and mitochondrion double-labeled transgenic line were put into the PGM containing 3-MA, and the mitochondria dynamics in the SCs within germinated pollen tubes were observed after 5 h. Treatment ②: the pollen grains from the double-labeled transgenic line were put into the PGM containing 3-MA, and the mitochondria dynamics in the SCs within pollen tubes were observed after 8 h. Treatment ③: the pollen grains from the double-labeled transgenic line were placed on PGM without 3-MA and first cultured for 3 h, then continue cultured in PGM with 3-MA for another 5 h. b,d,f, The representative picture from treatment ① (b), treatment ② (d) and treatment ③ (f) showed that autophagy inhibitor 3-MA could not effectively interfere with PME to cause mitochondrial accumulation. c, The number statistics of mitochondria (Mt) in the SCs treated with 0 mM, 1 mM, 2.5 mM and 5 mM 3-MA after 5 h (treatment ①). e, The number statistics of mitochondria in the SCs treated with 0 mM and 2.5 mM 3-MA after 8 h (treatment ②). g, The number statistics of mitochondria in the SCs treated with 0 mM and 2.5 mM 3-MA after 5 h (treatment ③). Results are from three independent experiments. The central lines represent the median, the boxes represent the interquartile range and the whiskers extend to the minima and maxima. Statistical significance is determined by one-way ANOVA (P > 0.05, no significance). Scale bars, 5μm.