Extended Data Fig. 1: Total nitrogen content in roots and shoots of OsLHT1a and OsLHT1b haplotypes under organic and inorganic fertilized soils.
a. Total nitrogen in root. b. Total nitrogen in shoot. n = 42 biologically independent samples (including 7 cultivars with 6 biological replicates per cultivar). Values represent means ± s.d. Two-way ANOVA showed significant effects of Genotype (F(1, 136) = 25.64, p < 0.0001), Fertilization (F(1, 136) = 20.52, p < 0.0001), and their interaction (F(1, 136) = 25.98, p < 0.0001) on roots. For shoots, significant effects of Genotype (F(1, 136) = 24.49, p < 0.0001), Fertilization (F(1, 136) = 13.89, p = 0.0003), and a marginal effect of Genotype:Fertilization interaction (F(1, 136) = 3.89, p = 0.051). Different letters above bars indicate significant differences between groups (p < 0.05) based on one-way ANOVA followed by Tukey’s HSD post-hoc test. A two-sided t-test was performed to compare the total nitrogen in the shoot and in the root of OsLHT1a and OsLHT1b grown in organic and inorganic N fertilization soils.
