Extended Data Fig. 8: RRS1 and BAR1 complementation lines.

a, Cell death phenotypes after infiltration of Pst DC3000D36E(AvrRps4). Images show leaves 1 dpi. Numbers indicate the number of leaves with cell death relative to the total number of infiltrated leaves. b, Pst DC3000 expressing AvrRps4 was sprayed on 5-week-old RRS1-S mutant complementation lines and bacterial colonies were counted at 3 dpi. c, Protein expression level of RRS1 complementation lines. d, Pst DC3000 expressing HopB1d was sprayed on five-week-old RRS1-S complementation lines and bacterial colonies were counted at 3 dpi. In b and d, statistical significance was tested by one-way ANOVA followed by Tukey’s HSD (different letters indicate p < 0.05). Mean values are shown with error bars ±SEM; n = 3 biologically-independent plants. e, Western blot showing protein expression level of BAR1 mutant complementation lines. Detailed statistical parameters are reported in Supplemental Table 6. All experiments were conducted at least three times with similar results.