Fig. 2: The CBSP screen reveals novel BIK1 substrates regulating immunity.
a, CBSP screen overview. b,c, AFM modelling of CBSP motifs. An overlaid model of all CBSPs with ipTM scores >0.75 (b) and the distribution of ipTM scores for WT or sequence-scrambled CBSP motifs (c). d, In vitro kinase assay using BIK1 and MBP-RBOHD-N. MBP-MBP served as a negative control. BIK1* indicates kinase-dead BIK1. Mean values are shown with error bars ±s.e.m.; P value from one-tailed t-test. a.u., arbitrary units. e, volcano plot of in vitro CBSP transphosphorylation by BIK1; n = 3 independent experimental replicates. Each point represents the mean ratio of autoradiographic signal for an MBP-CBSP protein versus a MBP control, as calculated in d. P values are derived from one-tailed t-tests. f–h, Volcano plots of infection assays using syringe-infiltrated Pst DC3000 (f), spray-inoculated Pst DC3000 Cor− (g) and drop-inoculated B. cinerea (h). Each point represents 1–3 independent experiments (n = 5–6 biologically independent plants per experimental replicate in f; n = 3–4 biologically independent plants per experimental replicate in g and h). Values are change in measured infection (either log10(c.f.u. cm−2 or lesion size; c.f.u., colony-forming units) of cbsp mutants versus corresponding Col-0 or Ler WT control. Each point represents the mean measured infection across all biological replicates for that genotype comparison. Linear mixed-effects modelling was used for statistical analysis. Detailed statistical parameters are reported in Supplementary Table 6. Models in f–h created with BioRender.com.
