Extended Data Fig. 7: sRNA profiling in root tissues.
From: Long-distance transport of siRNAs with functional roles in pollen development
a, Principal component analysis (PCA) of sRNA sequencing samples in roots. b, Abundance of global sRNAs from 21-24-nt in roots. c, Percentage of NRPD1-dependent sRNA abundance across different sizes ranging from 20-24-nt. d, Venn diagram displaying the overlap of root siRNA clusters (9085 clusters) and pollen PMsiRNAs (169 clusters). e, Number of root siRNA clusters mapping to TEs (n = ) and genes (n = ). f, Distribution of siRNA clusters based on length. Significance was tested using a two-sample proportion test: ***, P < 0.001; ** P < 0.01; ns, not significant. g, Percentage of root siRNA clusters mapping to different TE families. Significance was tested using Fisher’s test by comparing 707 root siRNA clusters that target PMsiRNAs in trans vs. random 707 root siRNA clusters, two-sided ***P-value < 0.001. not assigned: repeats not assigned to any TE family; no TEs: clusters not mapped to TEs. h, Motifs identified from 707 root siRNA clusters. E-value < 0.05: significant. i, Abundance (RPM value) of root siRNAs detected in leaves. Means of two biological replicates were computed. siRNAs were mapped to genes (left, n = 72), 2 kb promoters of genes (middle, n = 89), and TEs (right, n = 436).Center lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. The asterisks mark statistically significant differences (***, P < 0.001). Statistical significance of differences was determined using the pairwise Mann–Whitney U test, two-sided; the exact P values are provided in the Source Data file.
