Fig. 4: Grafting does not restore DNA methylation at PMsiRNA loci in microspores. | Nature Plants

Fig. 4: Grafting does not restore DNA methylation at PMsiRNA loci in microspores.

From: Long-distance transport of siRNAs with functional roles in pollen development

Fig. 4: Grafting does not restore DNA methylation at PMsiRNA loci in microspores.The alternative text for this image may have been generated using AI.

a,b, Metagene plots showing global DNA methylation levels of genes (a) and TEs (b) at CG, CHG and CHH positions. c, Examples of CHH methylation in microspores at four PMsiRNA loci of indicated genotypes. d, Boxplots showing methylation levels of all PMsiRNA loci (n = 169) of the indicated genotypes. Means of two biological replicates were computed. Centre lines show the medians; box limits indicate the 25th and 75th percentiles; and whiskers extend 1.5× the interquartile range from the 25th and 75th percentiles. The asterisks mark statistically significant differences (***P < 0.001). Statistical significance of differences was determined using the pairwise two-sided Mann–Whitney U-test; exact P values are provided in the source data. kb, kilobase.

Source data

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