Extended Data Fig. 9: In vitro dicing assay of DCL4 and DCL4-DRB4 complex towards RNA substrates with different lengths. | Nature Plants

Extended Data Fig. 9: In vitro dicing assay of DCL4 and DCL4-DRB4 complex towards RNA substrates with different lengths.

From: Molecular basis of DRB4-assisted long RNA processing and 21-nucleotide siRNA biogenesis by DCL4 in plants

Extended Data Fig. 9: In vitro dicing assay of DCL4 and DCL4-DRB4 complex towards RNA substrates with different lengths.

a, The sequences of three RNA duplexes used in this assay. b, In vitro dicing assay of DCL4 and DCL4-DRB4 complex to process RNAs with different lengths (30/32-nt, 55/57-nt, and 107/109-nt). The schematic view of the RNA length is shown on the right. The cutting sites on RNA are denoted by triangles. The in vitro assay was repeated at least three times independently with similar results.

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