Extended Data Fig. 1: Protein purification and in vitro activity assay.

a, The purification of Arabidopsis DCL4 (left) and DCL4-DRB4 complex (right). b, In vitro dicing assay of DCL4 and DCL4-DRB4 complex showing that DCL4-DRB4 has a stronger activity than DCL4 alone. The in vitro assay was repeated at least three times independently with similar results.