Extended Data Fig. 7: Cryo-EM image processing workflow of DCL4-DRB4CTD-dsRNA complex. | Nature Plants

Extended Data Fig. 7: Cryo-EM image processing workflow of DCL4-DRB4CTD-dsRNA complex.

From: Molecular basis of DRB4-assisted long RNA processing and 21-nucleotide siRNA biogenesis by DCL4 in plants

Extended Data Fig. 7: Cryo-EM image processing workflow of DCL4-DRB4CTD-dsRNA complex.

a, A representative cryo-EM image of DCL4-DRB4CTD-dsRNA complex. b, Flowchart of cryo-EM data processing. c, 2D class averages. d, Gold-standard Fourier shell correlation (GSFSC) of the final map. e, Angular distribution heat map calculated in cryoSPARC for particle projections contributing to the local resolution. f, Cryo-EM maps showing the fitting of the representative protein and RNA regions.

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