Extended Data Fig. 1: Schematic representation of the wild-type and the Δnad9 mitochondrial genomes.

The region from nucleotide position 331,000 to 338,000 (based on the Nicotiana tabacum reference genome; NC_006581) is shown. a, Map of the nad9 region in the wild-type mitochondrial genome. The nad9 coding sequence is located between nucleotide positions 336,111 and 336,683. The nad9-specific primers P1 and P2 were used to detect nad9 transcripts. b, Map of the corresponding genomic region in the mitochondrial genome of the Δnad9 mutant. A 3,809-nucleotide segment of the mitochondrial genome has been deleted (indicated by red arrowheads), leading to the complete loss of the nad9 gene. To detect the deletion, primers P3 and P4 were used that result in amplification of a short 325 bp fragment.