Extended Data Fig. 4: Presence of nad9 transcripts in root tissues of PMt plants.

RNA was extracted from the root tissues of wild-type, Δnad9, and PMt plants. atp9 and nad9 transcripts were co-amplified using gene-specific primers (see Methods), with atp9 serving as an internal control. nad9 transcripts are detected in all samples except for the Δnad9 mutant. RT-PCR followed by gel electrophoresis were performed once per sample.