Extended Data Fig. 2: Time-course profiling of base editor activity and mutation accumulation. | Nature Plants

Extended Data Fig. 2: Time-course profiling of base editor activity and mutation accumulation.

From: Mapping the zygote-to-adult developmental cell phylogeny in Arabidopsis thaliana reveals a three-cell rule of branching

Extended Data Fig. 2: Time-course profiling of base editor activity and mutation accumulation.

(a) Expression of the base editor in different developmental stages and organs. The base editor and GFP are linked by 2A peptide and under the driven of SlRPS5a promoter. 12 plants were analyzed with representative images of each organ shown. Images were captured by ZEIZZ LSM880 confocal microscopy. Bar = 100 µm. (b) mRNA expression level of the base editor in different stages and organs. 12 independent SMALT lines were analyzed. House-keeping gene ACTIN serves as an internal control. (c) Time-course mutation analysis. 6 rosette leaves (RL1 to RL6) were collected at the second, forth, and tenth week as indicated. The inflorescence (INF) and siliques (SQ) were also collected as representatives of reproductive organs. White arrowheads indicate collected samples. Bar = 1 cm. 4 individuals (Plant-A, -B, -C, -D) were sampled. For each sample, all sequenced barcodes were counted. Violin plots show mutation number per barcode in each sample. Blue curves indicate LOESS-smoothed fitted values of mutation number in vegetative and reproductive organs, respectively, with shaded regions representing the 99% confidence interval of the fit.

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