Fig. 1: Protocol of scalp acupuncture stimulation and exercise activity in rotarod test.

a Protocol of rotarod test and intervention. In SP8-Ap, scalp acupuncture stimulation was performed three times a week at 24 and 25 weeks. Each stimulation was followed by rotarod test. b Points of scalp acupuncture, Bai-Hui (GV20) and Yintáng (Ex-HN3). c Pictures of a mouse running on the rotating rod machine and the speed of rotating rod machine at 10 rpm in pre-training. rpm, resolutions per minute. d The speed of rotating rod machine in rotarod test. rpm, resolutions per minute. SP8-Ap, senescence-accelerated prone mice 8 received acupuncture stimulation; SP8-C, senescence-accelerated prone mice 8 without acupuncture stimulation; SR1, senescence-accelerated resistant mice 1 without acupuncture stimulation.