Table 5 RT-qPCR primer sequences for target validation

From: AI-aided identification of dual-purpose therapeutic targets PRPF19 and MAPK9 in hepatocellular carcinoma and cellular senescence

Gene

Primers ID Sequence

 

PRPF19

PRPF19-qPCR-F

GAAGCCAGAAGAGCTCAGCA

 

PRPF19-qPCR-R

TTTTTATCCGCCCCACCAGT

HRAS

HRAS-qPCR-F

ACGCACTGTGGAATCTCGGCAG

 

HRAS-qPCR-R

TCACGCACCAACGTGTAGAAGG

MAPK3

MAPK3-qPCR-F

TGGCAAGCACTACCTGGATCAG

 

MAPK3-qPCR-R

GCAGAGACTGTAGGTAGTTTCGG

MAPK9

MAPK9-qPCR-F

TACGTGGTGACACGGTACTACC

 

MAPK9-qPCR-R

CACAACCTTTCACCAGCTCTCC

RPS6KA3

RPS6KA3-qPCR-F

CGAGGTCATACTCAGAGTGCTG

 

RPS6KA3-qPCR-R

ACTGTGGCATTCCAAGTTTGGCT

MAPKAPK5

MAPKAPK5-qPCR-F

GCCCTGGATAATGTGCTGCCTT

 

MAPKAPK5-qPCR-R

GAGGCTGACTTTCAGATCCTGG

TOP2B

TOP2B-qPCR-F

GGTCAGTTTGGAACTCGGCTTC

 

TOP2B-qPCR-R

AGGAGGTTGTCATCCACAGCAG

Beta-Actin

b-actin-qPCR-F

CACCATTGGCAATGAGCGGTTC

 

b-actin-qPCR-R

AGGTCTTTGCGGATGTCCACGT

  1. R: reverse sequence, F: forward sequence.
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