Fig. 5: Inflammatory gene expression in HMC3 FAM49B-KO cells. | npj Aging

Fig. 5: Inflammatory gene expression in HMC3 FAM49B-KO cells.

From: Age-related nigral downregulation of the Parkinson’s risk factor FAM49B primes human microglia for inflammaging

Fig. 5: Inflammatory gene expression in HMC3 FAM49B-KO cells.The alternative text for this image may have been generated using AI.

a TLR genes, JUN and NFKB1 expression in HMC3 cells. b IL8 expression in HMC3 cells, c IL8 expression is triggered by TNFα stimulation. FAM49B-KO cells react significantly more to this stimulation. d The expression of IL8 is significantly ameliorated by NFkB inhibitor IV treatment in HMC3 FAM49B-KO cells. e Western blot and quantification of WT vs. FAM49B-KO HMC3 cells showing upregulation of Jun phosphorylation. f Circos plot correlating the top 15 contributing differentially expressed genes to common inflammatory pathways (data are presented as mean ± SEM and were analyzed using multiple t-tests in (a) and unpaired t-test in (b, c, e), and two-way ANOVA (d). *p < 0.05, ***p < 0.001).

Back to article page