Table 2 Intestinal morphology and intestinal stem cell markers in wild-type C57BL/6J and TNFα-/- mice

From: TNFα is a trigger of aging-associated liver inflammation in mice

Parameter

Groups

4 months

24 months

BL6b

BL6

TNFα-/-

Xylose large intestine (µmol/cm)

0.023 ± 0.003

0.019 ± 0.003

0.017 ± 0.001

Villi length (µm)

259 ± 7.8

285 ± 9.2

300 ± 19

Crypt depth (µm)

114 ± 8.5

101 ± 8.7

131 ± 13c

Villi width (µm)

77.2 ± 1.2

92.4 ± 2.7

100 ± 7.3

Tert mRNA expressiona

(% of young BL6 mice)

100 ± 21

66.5 ± 6.1

99.7 ± 11c

Lgr5 mRNA expressiona

(% of young BL6 mice)

100 ± 28

74.0 ± 7.7

122 ± 25c

Cd1d mRNA expressiona

(% of young BL6 mice)

100 ± 26

116 ± 18

110 ± 17

Nk1.1 mRNA expressiona

(% of young BL6 mice)

100 ± 38

116 ± 21

95.8 ± 13

  1. Values are means ± standard error of means. 4-month-old mice: n = 6–8, 24-month-old mice: n = 8–10 p-values were determined by unpaired t-test.
  2. BL6 wild-type C57BL/6J, Lgr5 leucine-rich repeat-containing G-protein coupled receptor 5, Tert telomerase reverse transcriptase.
  3. aSmall intestinal tissue.
  4. bShown for comparison.
  5. cp < 0.05 compared to 24 months old BL6.
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