Fig. 2: Phenotypic diversity of Segatella isolates.
From: Segatella clades adopt distinct roles within a single individual’s gut
A Scanning electron microscopy (SEM) images of select Segatella isolates, cultured in Schaedler broth until early stationary phase. Isolates names and corresponding clades are labeled on the top. B Quantification of biofilm formation by select Segatella isolates using crystal violet staining. Optical density at 590 nm was measured. Isolates are colored by the clades they belong to (n = 6 for each isolate, error bars showing the standard deviations). Dark and light gray bars denote the genomes that with or without T9SS present, respectively. C Crystal violet staining readings (OD590) of isolates with and without T9SS in the genomes. Statistical significance was calculated by two-tailed Mann-Whitney test (****p ≤ 0.0001, n = 78 for both groups). D Minimum Inhibitory Concentration (MIC, μg/mL) ranges of antibiotics on select Segatella isolates belong to different clades, as indicated by the color bars. The yellow dots denote the predicted antibiotic resistances as shown in (C). The > symbols indicate that no growth inhibition was observed within the concentration range we tested for the antibiotic.
