Fig. 1: Schematic representation of the experimental setup used throughout this study. | npj Biofilms and Microbiomes

Fig. 1: Schematic representation of the experimental setup used throughout this study.

From: Microfluidics unveils role of gravity and shear stress on Pseudomonas fluorescens motility and biofilm growth

Fig. 1

a The system is composed by a syringe pump, a commercial microfluidic channel (z = 0.4 mm, x = 17 mm, and y = 3.8 mm), and a tank of collection; details about components are reported in the method section. Bacterial were inoculated in stagnant conditions for 2 h before initiating flow imposing a fixed flow rate by the syringe pump. b The cartoon reports the microfluidic channel where sample was imaged in bright field time lapse at five different focal planes: channel bottom wall z1 (0 μm), z2 (100 μm), half-height channel z3 (200 μm), z4 (300 μm), channel top wall z5 (400 μm). CLSM z stack were acquired after sample staining, at the end of each experiment for bottom and top walls.

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