Fig. 1: Examination of response of salivary inoculum in chemically defined media (CDM) to the addition of Tryglysin A (TryA), a control peptide that does not affect growth or quorum sensing (reverse SHP/revSHP), or PBS.

Concentrations of TryA are indicated in the legend below graphs and correspond to the respective symbol types. Statistical significance compared to PBS control condition as indicated on a–c was determined using a One-way ANOVA with Tukey’s Multiple Comparisons Post-test; *p < 0.05; ***p < 0.005; ****p < 0.0001. Statistical significance is color-coded to match the corresponding figure legend. a Growth curve of salivary inoculum in CDM over time, with statistical significance shown for hours 8 through 24, if values are not indicated for these time points comparisons were non-significant. This experiment was performed three times with similar results. b Examination of pH of salivary inoculum in CDM over time, with statistical significance shown for hours 8 through 24, if values are not indicated for these time points comparisons were non-significant. This experiment was performed four times with similar results. c Separate experiment examining CFU/mL of salivary inoculum in CDM over time. If statistical values are not indicated, they are non-significant. This experiment was performed twice. d Jaccard PCoA plot of metagenomics results from samples presented in e. e Speciation as determined via shotgun metagenomics for samples grown in the presence of PBS, 1 µM TryA, or 1 µM reverse SHP peptide in CDM for 24 h. Relative abundance is plotted for each sample. Species detected are indicated by the legend beside the graph. f Composition of bacterial and fungal phyla detected in human pooled saliva from Norway by shotgun metagenomics and ITS sequencing.