Fig. 4: Punicalagin disrupted multiple biological pathways in the transcriptome of the MRSA strain MW2. | npj Biofilms and Microbiomes

Fig. 4: Punicalagin disrupted multiple biological pathways in the transcriptome of the MRSA strain MW2.

From: Exploiting strong synergies between punicalagin and cefoperazone to combat methicillin-resistant Staphylococcus aureus infections

Fig. 4

A Volcano plot of up- and down-regulated genes due to the treatment of MRSA strain MW2 with punicalagin (PA) at 10 μg/mL. B Pathways impacted by PA in the transcriptome of the MRSA strain MW2 in the presence of PA at 10 μg/mL. The color represents the significance (−log10 P value) of the changes. The Gene Ratio denotes the enrichment factor, i.e., the ratio of the number of differentially expressed genes to the total number of genes in a pathway. The size of the circle represents the number of differentially expressed genes mapped to the indicated pathway. C The diagram shows the pyrimidine metabolism pathway that was significantly affected by PA. D qRT-PCR verification of the expression of select genes, including carbamoyl phosphate synthase A (carA) and pyrB, pyrC, pyrE, and pyrF, that were significantly repressed by PA as detected by RNAseq. E correlations between qRT-PCR and RNAseq. The number represents the mean relative expression (or fold changes ± SD) of four replicates.

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