Fig. 2: Analysis of type 3 fimbriae production in K. pneumoniae in the presence of JT71. | npj Biofilms and Microbiomes

Fig. 2: Analysis of type 3 fimbriae production in K. pneumoniae in the presence of JT71.

From: Chemical inhibition of MrkH-dependent activation of type 3 fimbriae synthesis and biofilm formation by Klebsiella pneumoniae

Fig. 2

a SDS-PAGE of whole-cell lysates and Western blot of MrkA from K. pneumoniae grown with or without 50 µM JT71. K. pneumoniae containing pMrk (mrkABCDF) was used to overexpress type 3 fimbriae. A type 3 fimbriae mutant (ΔmrkA) was used as a negative control. b Hemagglutination assay showing the lowest bacterial concentration (cfu/mL) required to agglutinate tanned sheep erythrocytes. Horizontal bars represent the mean, and data points shown are biological replicates from one representative experiment of two independent experiments performed. Statistical analysis was performed using an unpaired two-tailed t test. c Representative images of hemagglutination reactions with K. pneumoniae at the indicated bacterial concentrations grown with or without 50 µM JT71. d Representative TEM images of K. pneumoniae immunogold-labelled with anti-MrkA antibodies. Cells were grown with or without 50 µM JT71. Immunogold particles (black dots) indicate surface-localised MrkA. Scale bars are shown.

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