Fig. 4: PaStL2 clears mature flow cell biofilms.
From: Two unrelated Pseudomonas aeruginosa phages require the exopolysaccharide Psl for infection
A The impact of phages on the initial attachment of PAO1 ∆wspF GFP+ bacteria to flow cells was evaluated by confocal microscopy. Phages were added to mature flow cell biofilms of PAO1 ∆wspF GFP+, and B 2 h or C 26 h post phage addition, confocal microscopy images of the biofilms were collected to determine whether the phages cleared the biofilms. Fluorescence due to GFP (bacteria) is shown in green, and HHL-TRITC (Psl) is shown in magenta. Typical images for each time point are shown. D The volume of bacteria in the flow cells for each time point and phage condition was determined using BiofilmQ, normalized to the volume of bacteria in the no-phage control at 2 h post phage addition. E The volume of Psl in the flow cells was similarly determined and normalized to the volume of Psl in the no-phage control at 2 h post phage addition. Data represent the means of results from 3 biological replicates (3 flow cells with 6 images collected per flow cell), and error bars represent standard deviation.
