Fig. 4: Analysis of the interaction between cecal microbes and neurometabolic pathway genes by feed restriction.

A The number of genes between the two groups was analyzed by Venn diagram. The blue circle represents Re group and the purple circle represents Co group. B Anosim method analysis of the gene clusters differences between the two groups. Horizontal is the group information, vertical is the distance information. “Between” refers to the difference between two groups, and the other refers to the difference within each group. The R values generally range from 0 to 1. The R value close to 1 indicates that the difference between groups is greater than the difference within groups, and an R value close to 0 indicates that there is no significant difference between and within groups. The reliability of statistical analysis is represented by p-value, and P < 0.05 indicates statistical significance. C, D Significant differences at the phylum and genus levels, respectively. Columns represent samples, and different colors represent different annotation information. Branch points represent different samples, different colors represent different groups, and the closer the branches, the more similar the samples. E Box plots showing the top 10 significantly different species between Co and Re. The horizontal axis is sample grouping. The relative abundance of the corresponding species is shown longitudinally. F The bar chart of LDA value distribution shows the significantly enriched pathways of differential microorganisms with LDA Score greater than the set value (default set to 2). The length of the bar chart represents the influence size of the differential functional pathway (i.e., LDA Score), and the color of the bar chart represents the respective groups. G Analysis of co-expression of top 20 key genes of neurometabolic pathways in the top 10 key microbial species and cecum tissues. In the heatmap, horizontal represents different genes, vertical represents significantly different microorganisms, and cluster analysis is performed on both horizontal and vertical data. Moreover, the color transformation from red to blue in the heat map represents different correlation coefficients, from positive correlation to negative correlation. The significant differences (“*”p < 0.05, “**” p < 0.01 and “***” p < 0.001) are indicated. H Construction of co-expression networks and analysis of interaction between flora and genes. The line between the two in the network diagram goes from red to gray, indicating that the correlation goes from strong to weak. The shape of each node from large to small represents the weight of the node in the correlation network from large to small.