Fig. 3: Phenotypic and genotypic variation in P. defluvii populations. | npj Biofilms and Microbiomes

Fig. 3: Phenotypic and genotypic variation in P. defluvii populations.

From: Competition between Pseudomonas species constrains ecological diversification in polymicrobial biofilms

Fig. 3

a Percentages of P. defluvii (brown) and P. brenneri (blue) phenotypic variants in each combination through the 18-day experiment. The numbers were obtained by phenotypic assessment of 92–96 isolated samples from each biological replicate in Congo red plates in every combination (a total of 5667 P. defluvii samples and 5710 P. brenneri samples were screened). The bars show the mean (n = 3) and SEM, and the individual values are presented in Table S3. PD P. defluvii, PB P. brenneri, BT B. thuringiensis, BD B. diminuta, PC P. communis. b Comparison of colony phenotypes of selected P. defluvii variants to their ancestor strain. The variants are presented according to the combination from which they were isolated. These images are representative of all the variants observed on Congo red plates. c Metagenomics analysis of samples of P. defluvii-derived variants obtained from different combinations after the 18-day experiment and analysis of genomes of selected evolved P. defluvii variants. Color gradient represents mutation frequencies across combinations in metagenomic samples (gray: 100%; light brown: 0%); gray boxes represent 100% frequency in isolated variants. DGC diguanylate cyclase. A detailed list of all the mutations is summarized in Table S4.

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