Fig. 4: Visualization of biofilm established and species intermixing over time in various communities.

Biofilm formation of the communities and single species biofilms (P. defluvii and P. brenneri) by confocal microscopy throughout the sampling days of the experiment (4, 10, 14, and 18 days). The strains P. defluvii (green) and P. brenneri (red) were tagged with GFP and mCherry, respectively. Lectin WGA (Alexa-Fluor 647) binding (yellow) represents B. thuringiensis and glycoconjugates. The scale bar represents 20 µm. PD P. defluvii, PB P. brenneri, BT B. thuringiensis, BD B. diminuta, PC P. communis.