Fig. 5: P. defluvii evolved variants display enhanced ability to co-exist with P. brenneri. | npj Biofilms and Microbiomes

Fig. 5: P. defluvii evolved variants display enhanced ability to co-exist with P. brenneri.

From: Competition between Pseudomonas species constrains ecological diversification in polymicrobial biofilms

Fig. 5

a Proportion between P. defluvii (brown) and P. brenneri (blue). 100,000 cells were counted for each biological replicate by flow cytometry from one of the PC slides (in total, 300,000 fluorescent cells). Data represent the mean and SEM (n = 3). The differences in percentages were tested as non-statistically significant with the PD:PB ancestor combination as a reference (Dunnett’s post-hoc test, p > 0.05), except for PD.V2 (p = 0.0195) and PD.V5 (p = 0.0101). b CFU/slide of all bacteria grown in each combination shown over time, representing biofilm formed on the PC slide. Data represent the mean (n = 3) and SEM, with three technical replicates each. CFU counts showed no significant difference between any combination for either strain (Tukey’s post-hoc test; p > 0.05). c CLSM images from some of the different dual combinations on day 10 of the experiment. P. defluvii and its derived variant (PD.V2) are shown in green, whereas P. brenneri and its derived variant (PB.V2) are shown in red. The images were taken with a 63x oil objective. The chosen images are representative of three biological replicates, recorded with at least six positions. The scale bar represents 20 µm.

Back to article page