Fig. 5: Evaluation of synthetic probes activity by crystal violet staining assay.

A The structure of photoaffinity probes, covalent probes, and negative probes. B Activity of probes on biofilm formation of P. aeruginosa PAO1. C Biofilm formation inhibition rates of different concentrations of 10d (1.25, 2.5, 5, 10, and 20 μM) for 24 h. D Biofilm formation inhibition rates of different concentrations of 10d-1 (1.25, 2.5, 5, 10, and 20 μM) for 24 h. All experiments are performed in triplicate, and data are presented as mean ± SD. ^aIC₅₀ = concentration of inhibitor needed to inhibit biofilm formation by 50%. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.