Fig. 1: Effects of a subchronic or chronic prucalopride treatment on corticosterone-induced anxiety- and depression-related behaviors.
Effects of a subchronic (A–G) or chronic (H–N) prucalopride (Pruca0.5, Pruca1.5) or fluoxetine (Flx) administration on corticosterone (CORT) -induced anxiety-like behaviors in the Elevated Plus Maze (EPM). Anxiety is expressed as mean time spent in seconds (A or H) or number of entries (B or I) in the open arms or as mean ratio of ambulatory distance in the center/total ambulatory distance (C or J). Effect of a subchronic (D) or chronic (K) prucalopride (Pruca0.5, Pruca1.5) or fluoxetine (Flx) administration on corticosterone (CORT)-induced depression-related behaviors in the Splash Test (ST). Depression-related behavior is expressed as mean duration of grooming in seconds (D or K). Effects of a subchronic (E, F) or chronic (L, M) prucalopride (Pruca0.5, Pruca1.5) or fluoxetine (Flx) administration on corticosterone (CORT) -induced anxiety and depression-like behaviors in the Novelty Suppressed Feeding (NSF) paradigm. Results are cumulative survival of animals that have not eaten over 10 min (E or L) or mean of latency to feed in seconds (F or M). Effects of a subchronic (G) or chronic (N) prucalopride (Pruca0.5, Pruca1.5) or fluoxetine (Flx) administration on corticosterone (CORT)-induced anxiety/depression-like phenotype. Test Z-values (Elevated Plus maze, Splash test and Novelty Suppressed Feeding) are then calculated by averaging individual Z-scores and averaged to obtain emotionality Z-score. Data are expressed as mean ± SEM (Veh/Veh n = 5, CORT/Veh n = 9, CORT/Flx n = 9–10, CORT/Pruca0.5 n = 9, CORT/Pruca1.5 n = 9). *p < 0.05; **p < 0.01; ***p < 0.001 versus appropriate group.
