Table 2 Plasmids used in this study

pEMPTY₀

E. coli - S. aureus shuttle vector. pEMPTY predecessor. No thermosensitive version. Amp^R Cm^R

⁶⁸

pEMPTY₀::sgRNA2

E. coli - S. aureus shuttle vector. It allows rapid and efficient plasmid curing in Staphylococcus aureus. Amp^R Cm^R

This study

pEMPTY₀::sgRNA_rsaE

E. coli - S. aureus shuttle vector. sgRNA targets the gene coding for RsaE. Amp^R Cm^R

This study

pEMPTY₀::sgRNA_rsaH

E. coli - S. aureus shuttle vector. sgRNA targets the gene coding for RsaH. Amp^R Cm^R

This study

pEMPTY₀::sgRNA_rsaI

E. coli - S. aureus shuttle vector. sgRNA targets the gene coding for RsaI. Amp^R Cm^R

This study

pFREE-Amp

Plasmid designed to remove plasmids from E. coli. CRISPR-Cas9-based curing. Amp^R

⁷⁸

pRN6680

pBluescriptΩ2.9-kb pMVN6 Smal-HindII [tetA(M)]. Amp^R Tet^R

⁶¹

pMAD

E. coli - S. aureus shuttle vector. The plasmid contains a thermosensitive origin of replication for Gram-positive bacteria and the bgaB gene that encodes a ß-galactosidase, a reporter of plasmid presence. Amp^R Ery^R

³²

pMAD_lic

pMAD modified plasmid for inserting DNA fragments using ligase-independent cloning. Amp^R Ery^R

³³

pMAD_lic::rsaE

pMAD_lic plasmid containing the regions needed for deletion of the rsaE gene. Amp^R Ery^R

This study

pMAD::rsaH

pMAD plasmid containing the regions needed for deletion of the rsaH gene. Amp^R Ery^R

This study

pMAD::rsaI

pMAD plasmid containing the regions needed for deletion of the rsaI gene. Amp^R Ery^R

This study

pMAD::mut.integrase

pMAD plasmid containing the regions needed for the mutation of the integrase gene of SaPIbov2. Amp^R Ery^R

This study

pCN40

E. coli - S. aureus shuttle vector. The plasmid contains the strong constitutive promoter P_blaZ. Amp^R Ery^R

⁶¹

pCN38

E. coli - S. aureus shuttle cloning vector. Amp^R Cm^R

⁶¹

pCN47

E.coli – S. aureus shuttle cloning vector. The plasmid contains a transcriptional terminator. TT Amp^R Ery^R

⁶¹

pCN51

E. coli - S. aureus shuttle vector. The plasmid contains the leaky constitutive promoter P_cad. Amp^R Ery^R

⁶¹

pCN51(Cm^R)

E. coli - S. aureus shuttle vector. Amp^R Cm^R pCN38 derivative: plasmid carrying the cadmium inducible promoter (Pcad), the polilynker region and the transcription terminators from the pCN51 plasmid.

This study

pCN51(Cm^R)::SaPIbov2 integrase

E. coli - S. aureus shuttle vector. Amp^R Cm^R pCN38::Pcad derivative. It contains the integrase from the pathogenicity island SaPIbov2.

This study

pAUR112

E. coli - S. cerevisiae shuttle vector. YAC, yeast artificial chromosome, high capacity vector. Amp^R URA3 AbA^R

TaKaRa

pAUR112::SaPIbov2::P_blaZ-cas system-sgRNA2-tetM

pAUR112 containing a modified SaPIbov2. sgRNA2. Amp^R URA3

This study

pAUR112::SaPIbov2::P_blaZ-cas system-sgRNA_rsaE-tetM

pAUR112 containing a modified SaPIbov2. sgRNA_rsaE. Amp^R URA3

This study

pAUR112::SaPIbov2::P_blaZ-cas system-sgRNA_rsaH-tetM

pAUR112 containing a modified SaPIbov2. sgRNA_rsaH. Amp^R URA3

This study

pAUR112::SaPIbov2::P_blaZ-cas system-sgRNA_rsaI-tetM

pAUR112 containing a modified SaPIbov2. sgRNA_rsaI. Amp^R URA3

This study

pCN51(Cm^R)::P_cad-terS_80α-TT

E. coli - S. aureus shuttle vector. pCN51 derivative: Ery to Cm resistance cassette substitution. It contains the gene coding the 80α small terminase subunit under the control of the pCN51 cadmium inducible promoter. Amp^R Cm^R

This study

pCN47::icaADBC

E. coli - S. aureus shuttle vector. pCN47 derivative. It contains the ica operon from the strong biofilm producer clinical strain 15981

This study

pCN47::bap

E. coli - S. aureus shuttle vector. pCN47 derivative. It contains the bap gene from the V329 mastitis isolate.

This study