Fig. 5: CLEC3A expression and tumor stroma interaction. | npj Breast Cancer

Fig. 5: CLEC3A expression and tumor stroma interaction.

From: Proteomic landscape of decellularized breast carcinomas identifies C-type lectin domain family 3 member A as a driver of cancer aggressiveness

Fig. 5

A Representative Western blot analysis of cell lysate (Lys), ECM, and dECM of MDA-MB-361, MDA-MB-231, and MDA-MB-468 cells seeded on collagen I, after transfection with pcDNA3 (Mock) or p3-CLEC3A plasmid (CLEC3A). Vinculin was used as control for cellular removal. B Representative stain of phalloidin (green) and tubulin (red) by immunofluorescence in cells grown on fibronectin. Nuclei are stained with DAPI (blue). Scale bar, 20 µm. C Representative WB analysis of mock and CLEC3A cells seeded on fibronectin. GAPDH was used as loading control.

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