Fig. 3: NF1 and CARS1 promote angiogenesis in human brain microvascular endothelial cells (HBMECs). | npj Genomic Medicine

Fig. 3: NF1 and CARS1 promote angiogenesis in human brain microvascular endothelial cells (HBMECs).

From: Identification and validation of CARS1 p.E712V and NF1 p.Q2002X in sporadic Moyamoya disease across 30 trio pedigrees

Fig. 3: NF1 and CARS1 promote angiogenesis in human brain microvascular endothelial cells (HBMECs).

A ~ E results of protein and phenotype experiments of NF1 gene; F ~ K results of protein and phenotype experiments of CARS1 gene. A Representative images of western blotting for NF1 and GAPDH. The statistical results of mRNA and protein expression were calculated. n = 3, **P < 0.01, ****P < 0.0001. B Representative images of western blotting for VEGF and GAPDH. Relative grey values of the protein bands are shown, and the statistical results of protein expression were calculated. n = 3. C Effect of silencing NF1 on the proliferative capacity of HBMEC. n = 3, *P < 0.05, **P < 0.01, ****P < 0.0001. D The tubule-like structure formation of HBMEC in the NF1-silenced group and the statistical comparison of the number of branch points and total capillary length. n = 3, *P < 0.05, **P < 0.01. E Effect of silencing NF1 on HBMEC cell migration. n = 3, *P < 0.05. F Representative images of western blotting for CARS1 and GAPDH. Relative grey values of the protein bands are shown, and the statistical results of protein expression were calculated. n = 3, ***P < 0.001, ****P < 0.0001. G Effect of different treatments of CARS1 on the relative proliferative capacity of HBMEC. n = 3, ****P < 0.0001. H Representative images of western blotting for GPx4 and GAPDH. Relative grey values of the protein bands are shown, and the statistical results of protein expression were calculated. n = 3, *P < 0.05. I Representative images of western blotting for VEGF and GAPDH. Relative grey values of the protein bands are shown, and the statistical results of protein expression were calculated. n = 3. J The tubule-like structure formation of HBMEC in the CARS1 treatment group and the statistical comparison of the number of branch points and total capillary length between treatment groups. K Effect of Effect of different treatments of CARS1 on HBMEC cell migration. n = 3, **P < 0.01, *P < 0.05, ****P < 0.0001. siNF1 NF1 silencing, siCARS1 CARS1 silencing, ev empty vector, CARS1-oe, CARS1 overexpression, CARS1-mut cells expressing the CARS1 p.E712V mutant, nc negative control.

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