Fig. 5: Endogenous Apo D expression was regulated by TAp73 in astrocytes.
From: Astrocyte-derived apolipoprotein D is required for neuronal survival in Parkinson’s disease
a–c Western blotting and statistical analysis of the expression of TAp73 and ApoD in TAp73 knockdown C6 cells and primary cultured VM astrocytes treated with MPP+ (100–200 μM). There was a dramatic decrease in the ApoD and TAp73 protein levels in sgTAp73 C6 cells and primary cultured VM astrocytes. n = 6. d–f Western blotting and statistical analysis of the protein levels of cleaved caspase-3 and caspase-3, Bcl-2 and Bax in sgTAp73 C6 cells and primary cultured VM astrocytes treated with MPP+ (100–200 μM). The changes in caspase-3 activity were increased accordingly compared with that of the controls. Similar results were found that the ratio of Bcl-2/Bax was decreased in sgTAp73 C6 cells and primary cultured VM astrocytes, compared with control. n = 6. g–h Western blotting and statistical analysis of the protein levels of SOD1 in sgTAp73 C6 cells and primary cultured VM astrocytes treated with MPP+ (100–200 μM). The expression of SOD1 protein levels was significantly reduced in MPP+-sgTAp73 C6 cells and primary cultured astrocytes, compared with that of controls. n = 6. Statistics were calculated using one-way ANOVA with a false discovery rate <0.05 b, c, e, f, h, Data were presented as mean ± SEM.
