Fig. 4: Voluntary exercise modulates the expression of ferroptosis-related markers via inhibition of ALOX12 in A53T mice. | npj Parkinson's Disease

Fig. 4: Voluntary exercise modulates the expression of ferroptosis-related markers via inhibition of ALOX12 in A53T mice.

From: Voluntary exercise alleviates neural functional deficits in Parkinson’s disease mice by inhibiting microglial ferroptosis via SLC7A11/ALOX12 axis

Fig. 4: Voluntary exercise modulates the expression of ferroptosis-related markers via inhibition of ALOX12 in A53T mice.

A Schematic diagrams of experimental time points. B Representative chemiluminescence images of ALOX12 protein band. C Comparisons of ALOX12/GAPDH ratio. D Comparisons of Alox12 mRNA level of mice in each group. E Representative images of colocalization of ALOX12 and Iba-1+ microglia in mice of each group (×40 objective, zoomed in 2). F Comparisons of fluorescence intensities of ALOX12 in mice of each group. G, H Comparisons of the Ptgst2, Gpx4 mRNA level of mice in each group. I Representative images of 4-HNE colocalized with Iba-1 in SN of mice in each group (×40 objective, zoomed in 4). J Comparisons of 4-HNE intensity in SN of mice in each group. K Representative images of TFRC colocalized with Iba-1 in SN of mice in each group (×40 objective, zoomed in 4). L Comparisons of TFRC intensity in SN of mice in each group. One-way ANOVA and Tukey’s post hoc tests. Each dataset is expressed as mean ± SD for n = 5. *P < 0.05; **P < 0.01; ***P < 0.001.

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