Fig. 4: EV-miRNA levels are altered in primary neuronal cells and EVs on treatment with rotenone.

(i) Primary midbrain cultures and (ii) Primary cortical cultures were treated with 25 and 60 nM rotenone, respectively, for 24 h and subsequently EVs were isolated and differential expression of miRNAs were checked in a total cells and b EVs by RT-qPCR. Asterisk (*), (**), (***), and (****) indicates values statistically significant from control; p value <0.05, <0.01, <0.001, and <0.0001 (respectively), SEM of three or four independent experiments, data were analyzed using one-way ANOVA, followed by Dunnett’s multiple comparisons test.